By L. G. Koss (auth.), Prof. Dr. med., Dr. rer. nat. Peter Pfitzer, Prof. Dr. med. E. Grundmann (eds.)
Progress in technology is usually promoted via a brand new approach. Diagnostic cytology, besides the fact that, built slowly over an entire century, regularly in differentiating malignant from benign cells from a number of tissues. The decisive step during this improvement used to be the extensive research of 1 neighborhood ization via Papanicolaou: the appliance of cytology for screening within the box of gynecologic oncology made it an stated strategy. therefore, fabrics inves tigated ahead of have been confirmed back on a bigger scale and new ones have been integrated into this system. the potential for a wide-range software of this diag nostic approach, which contains a low hazard for the sufferer and is least expensive, attracted the experts of many fields. one of many difficulties which has resulted is the coordination of educating and caliber coverage for a wide crew of individuals from diverse fields and with diverse pursuits and whose event in morphology varies. during this quantity normal difficulties of cytology are dis stubborn, as is the query "who is a clinical cytologist?" schooling and coaching are the themes of the contributions by means of Coleman, Holzner, Jenny, Koss and Muller, hide ing the location within the eu neighborhood, Germany, Austria, Switzerland, and the united states. a distinct contribu tion by means of Lange matters the placement of cytotechnologists, paramedicals vital for cytologic screening programs.
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The superimposition of cells in cytologic preparations makes reading of the immunocytologic reactions difficult. Recent Results in Cancer Research, Vol. 133 © Springer-Verlag Berlin· Heidelberg 1993 48 P. DaIquen et aI. 5. The provenance of cytologic specimens from different sites calls for special preparatory methods because of different content of cells and proteins. 6. The quality of specimens varies considerably with the technical skill of both the laboratory and the clinician who has sent the specimens.
Interpretation of the results is easier and less time consuming as the section area is small and some histologic patterns such as tubuli are preserved. 2. Up to 20 incubations can be performed on one sample. 3. Loss of the standard smear for the conventional cytologic diagnosis is avoided. ' Immunocytochemistry 51 4. The paraffin blocks are easy to store (Kung et al. 1990). Several major disadvantages, however, stand against it: 1. The main disadvantage is that this technique is only applicable to material containing a sufficient number of the relevant cells.
The risk of potentially fatal herpes infection of the neonate is most likely to follow primary infection of the mother in the first trimester of pregnancy. Infection of the skin and mucus membranes by HSV types 1 and 2 is characterised clinically by the formation of small vesicles in the epithelial layers. The vesicles contain an inflammatory exudate in which multinucleate giant cells may be found. The vesicles are often painful and may ulcerate and become secondarily infected. Both primary and secondary infections may be accompanied by fever and malaise.