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Unconsciousness will occur within 30 sec. 4. Verify death by lack of cardiac pulse and fixed and dilated pupils prior to carcass disposal. 6) 1. Weigh the animal and calculate dose of sodium pentobarbital at 60 mg/kg. 2. Restrain the animal. 3. Administer sodium pentobarbital intraperitoneally. 4. Verify death by lack of cardiac pulse and fixed and dilated pupils prior to carcass disposal. 4 Copyright C 2006 by John Wiley & Sons, Inc. 7) 1. Anesthetize the animal. 2a. Decapitate the animal by guillotine (rats) or with a sharp pair of scissors (mice).

Collect the blood that flows into and around the microhematocrit tube into a centrifuge tube. 5. Remove the tube and apply digital pressure over the eye to prevent hematoma formation. 4) 1. Anesthetize the hamster. Place the animal in dorsal recumbency. 2. Insert needle just below and slightly to the left of the xiphoid cartilage at the base of the sternum at an angle of 30◦ . 3. Advance needle and gently aspirate until blood begins to flow. If the procedure is terminal, up to 3 ml can be collected.

Pp. 261-301. John Wiley & Sons, Chichester, England. E. ) 1994. , ACLAM Series, pp. 104-105. Academic Press, San Diego. J. J. 1987. Anesthesia and surgery of laboratory animals. E. ) pp. 989-1017. B. Saunders, Philadelphia. K. L. 1997. Anesthesia and analgesia in rodents. F. K. J. J. 165200. Academic Press, San Diego. 5 Proper identification of animals plays an important role in research, whether animals are being used as experimental subjects, for breeding purposes, or as a source for tissues, cells, or fluids.

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